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KMID : 0358320080490020160
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Korean Journal of Urology
2008 Volume.49 No. 2 p.160 ~ p.167
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Changes in Corpus Cavernosum after Partial Bladder Outlet Obstruction in Rat
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Mo Kyo-Ik
Lee Hyung-Il
Lee Kyung-Seop
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Abstract
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Purpose: Abnormalities of the relaxation and contraction of the corpus cavernosum can lead to erectile dysfunction. Therefore, we induced a partial bladder outlet obstruction(PBOO) in male rats, and investigated the mechanisms of penile dysfunction with endothelial nitric oxide synthase(eNOS), vascular endothelial growth factor(VEGF), endothelin-1(ET-1), and apoptosis of peri-vascular smooth muscle and connective tissue cells in the corpus cavernosum.
Materials and Methods: PBOO was induced in 13 Sprague-Dawley rats by placing a 25 gauge needle sheath around the urethra, then ligating the bladder neck with a 3-0 suture. Three week after surgery, distal penile tissues were dissected for immunohistochemical staining, immunoblotting, and TUNEL staining.
Results: The expression of eNOS and VEGF were significantly decreased, whereas the expression of ET-1 and apoptosis of perivascular smooth muscle and connective tissue cells were significantly increased in the corpus cavernosum.
Conclusions: The significant increase of ET-1 and apoptosis along with decreased eNOS and VEGF could mediate erectile dysfunction. (Korean J Urol 2008;49:160-167)
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KEYWORD
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Partial bladder outlet obstruction, Endothelin-1, Endothelial nitric oxide synthase, Vascular endothelial growth factor, Apoptosis
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